Assessment of gelatin and alginate hydrogels for the development of bioinks

  • Matías F. Escribá García Lab3Bio (Laboratorio de Biomateriales, Biomecánica y Bioinstrumentación), Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín (UNSAM), San Martín, Buenos Aires, Argentina.
  • Ana Carolina Heindenreich Lab3Bio (Laboratorio de Biomateriales, Biomecánica y Bioinstrumentación), Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín (UNSAM), San Martín, Buenos Aires, Argentina.
  • Mercedes Pérez Recalde Lab3Bio (Laboratorio de Biomateriales, Biomecánica y Bioinstrumentación), Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín (UNSAM), San Martín, Buenos Aires, Argentina. CONICET.
  • Ignacio E. Ruiz Arias Lab3Bio (Laboratorio de Biomateriales, Biomecánica y Bioinstrumentación), Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín (UNSAM), San Martín, Buenos Aires, Argentina.
  • Ana González Wusener Instituto de Investigaciones Biotecnológicas (IIB-UNSAM), San Martín, Buenos Aires, Argentina. CONICET.
  • Élida B. Hermida Lab3Bio (Laboratorio de Biomateriales, Biomecánica y Bioinstrumentación), Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín (UNSAM), San Martín, Buenos Aires, Argentina. CONICET.

Abstract

Hydrogels of naturally occurring polymers are a good choice for the development of bioinks; they allow cell viability and a good performance during printing. In this work, mixtures of different proportions of gelatin and alginate were prepared and their elastic modulus (G’) and loss modulus (G’’) were measured as a function of time. These mixtures were employed as inks for printing, using a 3D bioprinter and different crosslinking agents. The compression modulus (E) of a 4-layer porous scaffold and the printability of a 1-layer porous scaffold, were correlated with the composition of the inks. The mixture of 15% w/v gelatin and 4% w/v alginate crosslinked with CaCl2 0,2M showed good rheological parameters and high printability. Keywords— biomaterials, hydrogels, 3D printing, rheological analysis.  
Published
2018-08-30
Section
Scientific articles