Synthetic polymeric scaffolds design and fabrication for wound healing treatment

  • Gabriela Rodriguez Laboratorio de medios e interfases
  • María Lourdes Gonzales Laboratorio de medios e interfases
  • Silvia Noemí Kozuszko Cátedra de Anatomía y fisiología patológicas
  • Juan Carlos Valdez Cátedra de Inmunología
  • Andrea Paola Rodriguez Laboratorio de medios e interfases

Abstract

Ulcers are a type of chronic wounds that are very susceptible to microbial infections of bacteria that produce biofilm, which makes them refractory to antibiotic and antiseptic treatment. It has been observed that treatment with Lactobacilus plantarum supernatant (SNLP) in venous ulcers, reduces wound healing time, significantly decreasing bacterial load, so it could be a good alternative as treatment for different types of chronic wounds. Polycaprolactone (PCL) is a biocompatible and biodegradable polymer, with excellent mechanical properties, whose effectiveness in dermatological applications has been widely demonstrated. In addition, with the electrospinning technique, PCL fibers can be created in micro and nanometric scale with a certain degree of porosity, very useful for different biomedical applications. Therefore, the purpose of this work is to manufacture polymeric nanomembranes incorporating the supernatant of Lactobacilus plantarum, as an antibiotic and healing substance to PCL nanofibers by means of the immersion technique. The method of manufacturing PCL nanomembranes was optimized by electrospinning using various polymeric supports. Different immersion methods were tested for supernatant incorporation, controlled release of the same was studied and its morphology was observed in a Scanning Microscope. The 10% concentration PCL nanomembranes are made up of uniform nanofibers of random distribution, with an average diameter of 169.06 ± 32.28 nm and an average pore size of 1266.74 ± 292.55 nm. According to these results, fiber morphology was adequate for encapsulation. On the other hand, these scaffolds show antimicrobial activity, however, SNLP release was almost instantaneous, so, we conclude that it is not a suitable biomaterial as a possible treatment for chronic wounds. Therefore, it is necessary to review the technique to incorporate and controlled release of this substance and apply coaxial electrospinning for hollow nanofibers filled manufacture with this bioactive substance as an alternative technique.

Author Biographies

Gabriela Rodriguez, Laboratorio de medios e interfases
Departamento de Bioingeniería, Universidad Nacional de Tucumán, INSIBIO-Consejo Nacional de Investigaciones Científicas y Técnicas, Tucumán, Argentina
María Lourdes Gonzales, Laboratorio de medios e interfases
Departamento de Bioingeniería, Facultad de Ciencias Exactas, Universidad Nacional de Tucumán
Silvia Noemí Kozuszko, Cátedra de Anatomía y fisiología patológicas
Facultad de Odontología, UNIVERSIDAD NACIONAL DE TUCUMAN
Juan Carlos Valdez, Cátedra de Inmunología
Facultad de Bioquímica, Quí́mica, Farmacia y Biotecnología, Instituto de Microbiología, Universidad Nacional de Tucumán, Tucumán, Argentina. .
Andrea Paola Rodriguez, Laboratorio de medios e interfases
Departamento de Bioingeniería, Universidad Nacional de Tucumán, INSIBIO-Consejo Nacional de Investigaciones Científicas y Técnicas, Tucumán, Argentina. 
Published
2021-01-06
Section
Scientific articles