Extracción de veneno en alacranes por estimulación eléctrica del telson

  • José N. Ricciardi Departamento de Ingeniería, Facultad de Ciencias Exactas y Naturales y Agrimensura, UNNE, Corrientes
  • Marcelo T. Sussini Departamento de Ingeniería, Facultad de Ciencias Exactas y Naturales y Agrimensura, UNNE, Corrientes
  • Eduardo J. Ricciardi Departamento de Ingeniería, Facultad de Ciencias Exactas y Naturales y Agrimensura, UNNE, Corrientes
  • Ana Maria Torres Departamento de Bioquímica, Facultad de Ciencias Exactas y Naturales y Agrimensura, UNNE, Corrientes

Abstract

Abstract— To be able to study the chemical composition of the scorpion venom of samples from the species Tityus trivittatus and T. conflues collected in the city of Corrientes (Argentina), it is requiered to do an extraction of an important amount of the venom using a method that allows the survival of the arachnids in captivity and that it is free of impurities. With this purpose, an electronic device has been developed for the extraction, based on the electrical stimulation of the telson muscle. The design consists of a timer which produces a unique continuous tension pulse of adjustable amplitude, intensity and duration (up to 30 volts, 100 miliampers and 1,8 seconds) that is applied with a metallic dissection clamp adapted to be used as bipolar electrodes to hold the alive specimen by its telson. The recollection of the venom (0,21-0,26 mg per specimen stimulated according to the specie) has been accomplished by switching on the device by a pulsating button. The venom is then empty dried for further analysis. This device proved to be very efficient as it didn´t affect the integrity of the specimens and permitted the obtention of pure venom every 15 days for each sample, fulfilling the prospect of the work. Keywords— scorpionic, neurotoxin, Tityus, arachnids.
Published
2017-03-01
Section
Scientific articles